解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (3 ): 349-352.doi: 10.3969/j.issn.0529-1356.2013.03.011

• 细胞和分子生物学 • 上一篇    下一篇

生殖细胞核因子及Oct-4在P 19细胞系诱导分化中的表达

王菊1,2  熊承良2  高启云1  李红钢2  王苗1  赵凯2  周宗瑶1*   

  1. 1.石河子大学医学院组织学胚胎学教研室，新疆 石河子 832002; 2.华中科技大学同济医学院计划生育研究所/生殖医学中心，武汉 430030
  • 收稿日期:2012-06-18 修回日期:2013-01-28 出版日期:2013-06-06 发布日期:2013-07-16
  • 通讯作者: 周宗瑶 E-mail:zongyaozh@163.com

Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line

WANG Ju 1, 2  XIONG Cheng-liang2  GAO Qi-yun1  LI Hong-gang2  WANG Miao1  ZHAO Kai2  ZHOU Zong-yao 1*   

  1. 1. Department of Histology and Embryology, School of Medicine, Shihezi University, Xinjiang Shihezi 832002, China;
    2. The Family Planning Institute, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2012-06-18 Revised:2013-01-28 Online:2013-06-06 Published:2013-07-16

摘要：

目的 体外培养未成熟畸胎瘤细胞（P19细胞）并诱导分化，检测生殖细胞核因子（GCNF）及Oct-4在P 19细胞诱导分化前后的表达。
方法 用全反式维甲酸（ATRA）诱导P 19细胞分化，通过间接免疫荧光染色和RT-PCR方法检测GCNF及Oct-4在P 19细胞诱导分化前后的表达。
结果 Oct-4在P 19细胞诱导前呈强阳性表达，诱导2 d后表达下降；而GCNF在P 19细胞诱导前呈阴性表达，诱导2 d后呈阳性表达。
结论 GCNF参与了恶性生殖细胞肿瘤的体外分化。GCNF可能是通过下调Oct-4基因的表达参与了恶性生殖细胞肿瘤的分化。

关键词: Oct-4, 生殖细胞核因子, P19细胞, 分化, 间接免疫荧光, 反转录-聚合酶链反应

Abstract:

Objective To detect the expressions of Oct-4 and germ cell nuclear factors(GCNF) before and after inducing P19
cells to differentiation. Methods The P19 cells were induced to differentiation with ATRA treatment.The immunofluorescence
staining and RT-PCR techniques were used to detect the expression of GCNF and Oct-4 before and after differentiation of mouse
P19. Results The intensities for Oct-4 immunofluorescence positive staining were stronger in non-differentiated P19 cells than
that in the differentiated P19 cells. The intensities for GCNF immunofluorescence positive staining were weaker in non-differentiate
P19 cells than that in the differentiated P19 cells. Conclusion GCNF is involved in the in vitro differentiation progress of
the malignant germ cell tumors. GCNF be inhibited through the Oct-4 gene expression in vitro differentiation of P19 cells.

Key words: Oct-4, Germ cell nuclear factor, P19 cell, Differentiation, Indirect immunofluorescence, RT-PCR